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. 2017 Dec;187(12):2698–2710. doi: 10.1016/j.ajpath.2017.08.005

Figure 4.

Figure 4

Effect of lipopolysaccharide (LPS) on myosin light chain kinase (MLCK) expression in mouse intestinal tissues in vivo. A: LPS administration (0.1 mg/kg, i.p.) caused a time-dependent increase in MLCK protein expression. Relative densitometry analysis was based on the upper band shown for MLCK expression. B: Increase in mRNA levels, as assessed by real-time PCR. C: LPS caused an increase in mouse MLCK mRNA levels in a pure population of intestinal epithelial cells (ileum) captured by laser-capture microdissection. D: LPS treatment (5-day experimental period) resulted in an increase in phosphorylated (p)-MLC in mouse intestinal tissues (ileum), as assayed by immunostaining and visualized by confocal microscopy (x, 40). E and F: Effect of i.p. LPS (0.1 mg/kg body weight) on mouse intestinal tissue tumor necrosis factor (TNF)–α (E) and IL-1β (F) expression over the 5-day experimental period. LPS treatment did not cause any change in the mouse tissue level of TNF-α or IL-1β. Data are expressed as means ± SEM (A–C, E, and F). n = 4 (B); n = 5 (C); n = 3 (D). ∗∗P < 0.01, ∗∗∗P < 0.001 versus control. Scale bar = 5 μm (D).