Figure 2.

The distribution of intracellular organelles retains its polarity in isolated human acini. A and B: Untreated freshly isolated human acini were formaldehyde fixed and immunostained with antibodies against trypsinogen (Trypsin; zymogen granules; yellow stain), calnexin (endoplasmic reticulum; red stain), and translocase of outer membrane 20 (Tom20; mitochondria; green stain). The signals were detected with Alexa Fluor 555–, Alexa Fluor 633–, and Alexa Fluor 488–conjugated secondary antibodies, respectively. Nuclei were stained with DAPI (blue). Images were analyzed under a confocal microscope. C and D: Higher magnifications of the boxed areas in A and B, respectively. Scale bars: 10 μm (A and B); 20 μm (C and D). DIC, differential interference contrast.