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. 2017 Dec;187(12):2811–2820. doi: 10.1016/j.ajpath.2017.08.014

Figure 2.

Figure 2

Pulmonary pathology after MtbΔsigH vaccination and simian immunodeficiency virus (SIV) infection. A–D: Hematoxylin and eosin staining of lung sections from a representative animal showing an entire lung section (A), with boxed areas in A shown as enlarged images of consolidation (B), vaccine-induced bronchus-associated lymphoid tissue (BALT) (C), and healthy lung tissue examples (D) corresponding to the boxed regions in A. E: Quantification of overall pathology as percentage of lung involvement. Multiple lung sections per animal were scored and quantitatively compared with those from Mtb/SIV–co-infected animals in total pathology score (F) and lymphangitis (G). H: Peripheral viral loads in vaccinated animals (color) and Mtb/SIV–co-infected animals (gray). Dotted line indicates the time of SIV infection. I:In situ hybridization demonstrating the presence of SIV-infected CD3+ T cells in the lungs of vaccinated animals. J: Enlarged inset image of an infected cell corresponding to the boxed area in I. Data are expressed as means ± SEM (E–H). ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001 using a t-test analyzing the mean values of all vaccinated animals versus Mtb/SIV–co-infected animals. Scale bars: 100 μm (I); 5 μm (J).