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. 2017 Dec 5;16:222. doi: 10.1186/s12934-017-0835-1

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Growth curve of the S. pastorianus strains CBS1483 (filled circle) and IMX1187 (Spcas9) (open circle). The strains CBS1483 and IMX1187 were strains were grown in complex medium (YPD) at 20 °C. Growth was monitored based on optical density at 660 nm (OD₆₆₀) measurements. The data plotted are average and standard deviation of three biological replicates