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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Immunobiology. 2017 May 16;222(8-9):900–912. doi: 10.1016/j.imbio.2017.05.011

Figure 9. Changes of cytokine expression in CD163-overexpressing THP-1 macrophages challenged with a double LPS stimulation and a CD163 antibody.

Figure 9

Quantification for IL-10 (A) and IL-1ra (B) protein concentration in THP-1 macrophages transfected with either a plasmid encoding for CD163 gene (pCD163) or the empty vector (pEmpty) and incubated with either anti-CD163 antibody (RM3/1) or its isotype control antibody at 4 (for IL-10) and 24 (for IL-1ra) hours after a second LPS stimulus. The protein concentration of each group was normalized to the control group (pEmpty), which was assigned a value equal to 1. N= 5–12 per group. *p<0.05 using student’s t-test.