Table 2. qPCR cycling parameters.

Program	fLuc		hLB2C1
	Temperature (°C)	Time	Temperature (°C)	Time
Initial Denaturation	95	10 min	95	10 min
Denaturation	95	10 sec	95	10 sec	45 cycles
Annealing	62	10 sec	64-60 touchdown (5 cycle delay, 2 °C Δ/cycle)	10 sec
Extension	72	10 sec	72	10 sec
Melting Curve	95	10 sec	95	10 sec
	65	60 sec	65	60 sec
	65-97	0.1 °C/sec	65-97	0.1 °C/sec