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. 2017 Oct 31;6(11):1263–1267. doi: 10.1021/acsmacrolett.7b00725

Figure 2.

Figure 2

(A, inset) Cryo-TEM image of HRP-loaded vesicles. (A, top) Schematic showing the HRP-catalyzed oxidation of DMB to its colored dimer product detected in the colorimetric assay. (A, bottom) Activity of the HRP-loaded vesicles against negative controls. (B) Cryo-TEM images of GOx-loaded vesicles using commercial GOx (top) and purified GOx (bottom). (C, top) Schematic showing the GOx-catalyzed oxidation of d-glucose to δ-glucono-1,5-lactone. Excess HRP in the external solution was then used to catalyze the oxidation of DMB using hydrogen peroxide product from the first step. (C, bottom) Activity of the GOx-loaded vesicles against negative controls. The error bars in A and C show the standard deviation from four repeats. All scale bars = 200 nm.