Figure 3.

H₂O₂ release rate decline at different protein concentration and safranin response. (a) Three different protein concentrations were used to monitor H₂O₂ release in RET-like conditions. Curves 1–3 correspond to 0.5, 0.25, and 0.125 mg of protein/ml protein in the measuring assay. Dashed lines show fitting of exponential decay function. (b) Half-life of exponential decay of H₂O₂ release rate at difference protein concentrations (n = 3–4 for each data point). (c) Safranin O fluorescence after addition of 5 mM succinate and 1 mM glutamate to 0.15 mg of protein/ml. Assay conditions are as shown in Figure 2(b), except 2 µM safranin substituted Amplex UltraRed and horseradish peroxidase in (c). Concentration of FCCP was 0.1 µM.