Table 1. RNAseq analysis of OXPHOS complex genes in B05 cerebellar tissue versus wild type tissue.
|
Downregulation of OXPHOS complex genes in B05 (log 2 fold change) |
|||||
| Complex I | Complex II | Complex III | Complex IV | ATPase | |
| vs Wt-5 wks | *Cox6b2 (1.77038) | Lhpp (0.641673) | |||
| vs Wt-12 wks | Ndufb2 (0.634449) | Sdhc (0.515247) | Uqcrh (0.335416) | Cox5a (0.404022) | Atp5g3 (0.342982) |
| Ndufb3 (0.457995) | Sdhd (0.477251) | Cox6a1 (0.514417) | Lhpp (0.780756) | ||
| *Cox6b2 (1.27923) | |||||
| Cox4i1 (0.368788) | |||||
| vs Wt-28 wks | |||||
|
Upregulation of OXPHOS complex genes in B05 (log 2 fold change) |
|||||
| Complex I | Complex II | Complex III | Complex IV | ATPase | |
| vs Wt-5 wks | |||||
| vs Wt-12 wks | Ndufa11 (-0.349446) | Uqcrq (-0.416653) | Cox7a2l (-0.328557) | Atp5j2 (-0.516911) | |
| Ndufb5 (-0.356859) | Cox7b (-0.326646) | ||||
| Ndufs5 (-1.67463) | |||||
| Ndufv2 (-0.552158) | |||||
| vs Wt-28 wks | Ndufa11 (-0.509067) | Sdhc (-0.498714) | Uqcr11 (-0.454752) | Cox7a2l (-0.583099) | Atp5j2 (-0.493386) |
| Ndufa6 (-0.507489) | Uqcrq (-0.650516) | ||||
| Ndufa7 (-0.480316) | |||||
| Ndufb10 (-0.4287) | |||||
| Ndufb7 (-0.502352) | |||||
| Ndufb8 (-0.599094) | |||||
| Ndufb9 (-0.434831) | |||||
| Ndufs8 (-0.450487) | |||||
Top table lists genes upregulated (P<0.01) in B05 compared to wild type at 5, 12 and 28 weeks. Bottom table lists genes downregulated (P<0.01) in B05 compared to wild type at 12 and 28 weeks. Complex genes were not downregulated at 5 weeks.
*Indicates Purkinje-cell enriched genes [42].