Table 1. Primers used for sequencing.
| Name | 5’-3’ | Strainsa |
|---|---|---|
| Specific primers for the target oprD gene | ||
| oprD_PCR F | CGTCGCTTCGGAACCTCAACTA | All strains |
| oprD_PCR R | GCCGTGACCTCGAACCTGA | All strains |
| oprD_seq F | CCTGAAGCTCGACGGCACCTC | All strains |
| oprD_seq R | TCGCCGTAGCCGTAGTTCTTA | All strains |
| oprD_seq F2 | CAGCCGCCTGTTCCCGCAGACC | All strains |
| oprD_seq R2 | GGTAGGCCAAGGTGAAAGTGTG | All strains |
| oprD_seq F3 | GCTGCTCCGCAACTACTATTTC | All strains |
| Specific primers for the insertion sequence | ||
| oprD_seq R4 | GGCGTGCCGTCGTTCATCACTG | 16/PN/12, 53/PN/13, 482/PA/15 |
| oprD_seq F5 | AGAAGTTGGTCGAGCGTGCG | 482/PA/15 |
| oprD_seq R5 | TGCTTGCCTTCGGTGAAGTG | 482/PA/15 |
| oprD_seq F6 | CCCGCTCGATACGGTGTATCC | 482/PA/15 |
| oprD_seq R6 | GGCGTTGGTGGTGTAGATCACC | 482/PA/15 |
| oprD_seq F7 | TTGATCACCAACTCAGCATCCG | 16/PN/12, 53/PN/13 |
| oprD_seq R7 | TGATGTCGGACGAGTTCAATCG | 16/PN/12, 53/PN/13 |
| oprD_seq R8 | CAACGTCGTTCCTTTACGCCCG | 16/PN/12 |
aIsolates used for sequence analysis of oprD gene: 4/PN/12, 10/PN/12, 13/PN/12, 16/PN/12, 25/PN/12, 33/PN/12, 37/PN/13, 53/PN/13, 10/PN/14, 7/PN/15, and 482/PA/15.