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. 2017 Dec 6;12(12):e0189172. doi: 10.1371/journal.pone.0189172

Table 4. Different OprD types and mechanisms of imipenem resistance detected among the P. aeruginosa isolates.

Imipenem resistance phenotype	Isolates	Alteration(s) of oprD nucleic sequence	Alteration(s) or amino acidic substitution(s) of OprD sequence^a	Effect on OprD protein
Susceptible strains	4/PN/12, 25/PN/12, 33/PN/12, 37/PN/13	Several polymorphisms	D₄₃N, S₅₇E, S₅₉R, E₂₀₂Q, I₂₁₀A, E₂₃₀K, S₂₄₀T, N₂₆₂T, A₂₆₇S, A₂₈₁G, K₂₉₆Q, Q₃₀₁E, R₃₁₀G, V₃₅₉L, ₃₇₂(VDSSSS-YAGL)₃₈₃	Full length type
	10/PN/14	Several polymorphisms	T₁₀₃S, K₁₁₅T, F₁₇₀L	Full length type
	7/PN/15	Several polymorphisms	S₅₇E, S₅₉R, V₁₂₇L, E₁₈₅Q, P₁₈₆G, V₁₈₉T, E₂₀₂Q, I₂₁₀A, E₂₃₀K, S₂₄₀T, N₂₆₂T, T₂₇₆A, A₂₈₁G, K₂₉₆Q, Q₃₀₁E, R₃₁₀E, A₃₁₅G, L₃₄₇M, ₃₇₂(VDSSSS-YAGL)₃₈₃, S₄₀₃A, Q₄₂₄E	Full length type
Resistant strains	10/PN/12, 13/PN/12	Several polymorphisms Deletion of 37 nt at position 1046 nt	D₄₃N, T₁₀₃S, K₁₁₅T, F₁₇₀L	Frameshift mutation, premature stop codon (shorter, nonfunctional protein)

	16/PN/12, 53/PN/13	Insertion of 1239 nt at position 215 nt		Premature stop codon and porin loss

	482/PA/15	Several polymorphisms Insertion of 1337 nt at position 428 nt	S₅₇E, S₅₉R, V₁₂₇L	Premature stop codon and porin loss

^aIn bold, mutations which determine a charge change

+/-: K₁₁₅T, V₁₂₇L, V₁₈₉T, A₂₆₇S, V₃₅₉L, Q₄₂₄E

-/+: S₅₉R, E₁₈₅Q, E₂₀₂Q, E₂₃₀K, T₂₇₆A, S₄₀₃A

+/neutral: P₁₈₆G, A₂₈₁G, R₃₁₀G, A₃₁₅G