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(A) Structure of O-mycoloylation probe O-AlkTMM (32). (B) Simultaneous metabolic labeling of M. smegmatis with 6-TreAz and O-AlkTMM followed by sequential SPAAC and CuAAC reactions to deliver different fluorophores to trehalose glycolipids and AGM (image reprinted with permission from ref. 104). Scale bar, 5 μm. (C) Chemical synthesis of O-AlkTMM using a 4-step trehalose desymmetrization approach.
