Table 1.
Primers used in quantitative polymerase chain reactions.
| Target gene | Transcript ID | Sequence | Temperature |
|---|---|---|---|
| Caspase 16 | AAEL005956-RA | 5′-TCCGCTATCTTCATATTGTATCCTTTG-3′ | 60 °C |
| Aedronc | AAEL011562–RA | 5′-CAACTTTCCAACTGCCTATAAATTGC-3′ 3′-CTCCACCGTATCGTTATTGTTCTTAG-5′ |
60 °C |
| Cathepsin-B | AAEL007585-RA | 5′-CAAAGCACTCCCTTCCATC-3′ 3′-CACGAGCGTCGAATGTATC-5′ |
57 °C |
| Niemann–Pick Type C-2 | AAEL015136-RA | 5′-GCACTCGTCCCAGCTGTAATG-3′ 3′-CACTGACCAGCGGATAGATGG-5′ |
57 °C |
| β-Actin | AAEL001928-RA | 5′-AAGGCTAACCGTGAGAAGATGAC-3′ 3′-GATTGGGACAGTGTGGGAGAC-5′ |
60 °C |