Fig. 5.

Treatment of human saphenous organ cultures with 10 μM K66 retarded neointima formation. Segments of human saphenous vein were subjected to organ culture in the presence of BrdU, and 10 μM K66 or vehicle control (0.1% DMSO) for 14 days, n = 6. a, EVG-stained sections of human saphenous vein organ cultures. b, Immunohistochemistry for intimal (upper panels) and medial (lower panels) α-smooth muscle cell actin (brown). c, Proliferation detected by immunofluorescence for incorporated BrdU (green). Nuclei are blue (DAPI) in lower panels. Arrows indicate positive cells. Scale bars represents 10 μm and apply to all panels. Dashed lines indicate intimal:medial boundaries. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)