A) Oil red O lipid staining of aortic sinus from the indicated mice following HFD treatment for 8 weeks at 22°C or 30°C housing, which quantification (right). B) Total body weight in mice after HFD. C) Ucp1−/−
Ldlr−/− mice fed HFD for 12 weeks and were housed at 22°C or 30°C and assayed for plaque development. D) Glucose tolerance tests (GTT) were performed on 22°C or 30°C housed Ldlr−/− and Apoe−/− mice following 8 weeks HFD. E) Ucp1−/−
Ldlr−/− and Ldlr−/− mice fed HFD for 8 weeks and housed at 22°C were assayed by GTT. F) Ucp1−/−
Ldlr−/− mice fed HFD were housed at 22°C or 30°C and assayed for GTT. All data are representative experiments, containing n≥5 animals per group and repeated in two or three independent times. Statistical analysis was performed by unpaired student T-test, * p≤0.05, **p≤0.01, ***p≤0.001.