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Cy3–Cy5 FRET efficiency of 1 μM 22/9/9 GC +(D-Arg)9 TIVA probe compared to 18/7/7 +(D-Arg)9 TIVA probe in 1× STE buffer with or without 1 eq of 30mer polyA target and/or photolysis. Average and standard deviation shown for three or more samples under each condition.