Fig. 8.
(A) Cy3–Cy5 FRET efficiency of 1 μM PS-22/9/9 TIVA probe in 1× STE buffer with or without 1 eq of 30mer polyA target and/or photolysis. (B) Pre-photolysis FRET efficiency of PS-22/9/9 probe in the presence of 1 eq of 30mer polyA RNA is enhanced in PBS with 10% FBS. Average and standard deviation shown for three or more samples under each condition.
