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. Author manuscript; available in PMC: 2018 Dec 6.
Published in final edited form as: Org Biomol Chem. 2017 Dec 6;15(47):10001–10009. doi: 10.1039/c7ob02353g

Fig. 9.

Fig. 9

(A) 24 h stability time course of 1 μM phosphorothioate-linked PS-22/9/9 (green) or phosphodiester-linked 22/9/9 (red) TIVA probes in 10% FBS. Cy3–Cy5 FRET efficiencies were measured by fluorimeter and then normalized against starting values. (B) 48 h stability time course of 1 μM PS-22/9/9 (bottom) or 22/9/9 (top) probes in 10% FBS using gel electrophoresis. At each time point a sample was mixed with a nuclease inhibitor and flash frozen. After 48 h, all samples were loaded onto a 20% acrylamide denaturing gel and eluted using 100 V for 120 min. Cy3 fluorescence is shown in green and Cy5 fluorescence in red.