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. Author manuscript; available in PMC: 2019 Jan 2.
Published in final edited form as: AIDS. 2018 Jan 2;32(1):1–10. doi: 10.1097/QAD.0000000000001674

Figure 3. Significant suppression of HIV-1 RNA and DNA using free-fed ART chow and rapid recrudescence after treatment withdrawal.

Figure 3

(A) Schematic of the experimental timeline. (B) Levels of HIV-1 p24 in plasma of untreated mice 6wpi were used to assign groups with equal plasma antigenemia prior to treatment commencement. (C) Levels of vRNA in plasma (left panel) and HIV-1 DNA in CD4+ enriched cells from spleen (right panel) in mice at 10wpi that had either received 4 weeks of ART-supplemented chow (ART FOOD) or normal mouse chow (MOCK). (D) Comparison of vRNA in plasma (left panel) or HIV-1 DNA in CD4+ enriched spleen cells (right panel) from mice at 12wpi that had either never received ART (MOCK) or that had received ART for 4 weeks and then had it withdrawn for 2 weeks to assess viral recrudescence (ART FOOD). Horizontal bars denote mean. Horizontal shading demarks LODs of 800 copies/ml (light grey) or 300 copies/ml (dark grey). ns=not significant, ***P<0.0001, ****P<0.0001 unpaired T-tests.