Serum was collected from the F2 generation of mice at 12 months of age, and then subjected to enzyme-linked immunosorbent assays for estradiol, progesterone, and testosterone. The graph represents the means ± SEM (n = 3–5) of serum levels of estradiol, progesterone, and testosterone in the F2 mice at 12 months (panels A–C). Asterisk (*) represents statistically significant differences from the vehicle control (p ≤ 0.05).