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. Author manuscript; available in PMC: 2018 Dec 5.
Published in final edited form as: Cell Metab. 2017 Oct 26;26(6):884–896.e5. doi: 10.1016/j.cmet.2017.09.024

Figure 7. AMPK- and DR-mediated longevity involves peroxisome function.

Figure 7

(A) and (B) Survival curves showing that CA-AAK-2 has no additive effect on the lifespan of drp-1;fzo-1 double mutant worms. (C) and (D) Inhibiting fatty acid oxidation with PHX partially suppresses CA-AAK-2-mediated longevity. (E) Peroxisome size in YA wild-type and eat-2(ad1116) mutants. Median and interquartile range of n=2174–3187 peroxisomes, *p<0.05 by t test. (F) DR by bacterial dilution maintains peroxisome density (% cell coverage) with age. Mean ± SEM of n=18–25 worms. **p<0.01, ****p<0.0001 by one-way ANOVA with Tukey’s multiple comparisons test. (G) and (H) prx-5 RNAi reduces DR-mediated longevity. (I) CA-AAK-2 does not alter peroxisome size in young adult (YA) stage worms. Median and interquartile range of n=879–918 peroxisomes. (J) Phenformin treatment from YA stage maintains peroxisome density between YA and day 8. Mean ± SEM of n=11–35 worms. ***p<0.001 by one-way ANOVA with Tukey’s multiple comparisons test. (K) and (L) prx-5 RNAi suppresses CA-AAK-2-mediated longevity. See Table S1 for lifespan statistics.