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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Reprod Toxicol. 2017 Oct 3;74:181–188. doi: 10.1016/j.reprotox.2017.09.010

Fig. 3.

Fig. 3

Effects of ITE on AhR protein levels of and CYP1A1 and CYP1B1 mRNA levels in HUVECs and HUAECs. Protein levels (A and B): Cells were treated with a single dose of ITE (1 μM) or DMSO (0 hr, the control) up to 48 hr. Proteins were subjected to Western blotting. Representative images of Western blotting are shown. Data normalized to GAPDH are expressed as means ± SEM fold of the control (n = 4–5). mRNA levels (C and D): Cells were treated with ITE (1 μM) or DMSO (0 hr, the control) for 24 or 48 hr, followed by RT-qPCR assay. Data are expressed as means ± SEM fold of the control (n = 3). One-way ANOVA was conducted followed by comparisons verse the respective control within each cell type. *Differ from the time 0 control within each cell type (p < 0.05).