Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 6;7:17071. doi: 10.1038/s41598-017-16897-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Ex vivo trans-scleral 2-photon imaging of structure and cells in distal aqueous drainage tract of GFP/mTomato mice. (A) Orthogonal image reconstruction showing td-Tomato (red) and GFP (green) lining an intrascleral plexus (ISP), collector channel (CC) and outer wall of Schlemm’s canal (SC) within scleral collagen (SHG; cyan). Vertical dashed lines: serial depths (z) of optical slices shown in b-i. Horizontal arrows: laser path. (B) Branching ISP lumen located z = 16.5 μm deep to the conjunctival epithelium. (C) At z = 22.5 μm, ISP connection to a CC is evident. (D) At z = 33 μm, the vertically orientated CC is clearly separate from ISP. (E) At 43.5 μm, the CC maintained its course but narrows. (F) At z = 54 μm, the CC merges with a different, deeper ISP (asterisk). (G) At 64.5 μm, the CC widens as it approaches SC. (H) At z = 75 μm, the CC opens into SC at a collector channel opening (CCO). (I) View inside SC wherein a mosaic of GFP and td-Tomato-positive cells populate the outer wall.