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. 2017 Dec 6;7:17071. doi: 10.1038/s41598-017-16897-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of Prox1-GFP expression in collector channels. (a–c) Whole mount (external corneal surface faces reader) of Prox1-GFP mouse eye labeled with anti-LYVE-1 antibodies. (a) Merged image shows LYVE-1-positive, Prox1-positive (orange/yellow with co-localization) limbal lymphatic vessels close to Schlemm’s canal (SC; green). SC was a ~125 μm wide circumferential band showing only Prox1-GFP-positivity. Arrowheads: region to be magnified in subsequent panels, (b–i). (b,e,h) Green channel image showing Prox1-GFP-positive structures, representing both distal aqueous drainage tract and limbal lymphatics. (c,f,i) Red channel image showing LYVE-1-positive structures only, representing limbal lymphatics but not SC or distal aqueous drainage tract. (d–f and g–i): 5X and 10X magnification respectively of region indicated by arrowheads in panel (a–c). Arrows: LYVE-1-positive, Prox1-positive limbal lymphatic vessels that are orange/yellow from co-localization. Prox1-GFP and LYVE-1 co-localize (orange/yellow) in limbal lymphatic vessels, but CC (boxes in g,h; inset in h; discontinuous expression) and SC express Prox1-GFP only.