Table 2.
Imaging modalities.
| Modality | Acronym | Color | Example | Represents |
|---|---|---|---|---|
| 1. Two-photon excitation fluorescence: | TPEF | |||
| a. Endogenous fluorophores | Red | td-Tomato | Plasma membrane | |
| VE-cadherin-td-Tomato | Endothelium | |||
| Green | GFP | Cytoplasm | ||
| Prox1-GFP | Endothelium of distal tract or lymphatics | |||
| b. Epifluorescence | Red | CD31 (aka PECAM1) | Endothelium | |
| F-actin (Alexa- 568-Phalloidin) | Contractile cytoskeleton | |||
| LYVE-1 | Lymphatic endothelium | |||
| VEGFR2 | Endothelium | |||
| c. Intravital fluorescence | Green | Hoechst 33342 | Nucleus | |
| siGLO | Nucleus | |||
| d. Autofluorescence | TPEF AF | Green | Elastin and fibrillar collagen | Elastin and fibrillar collagen (e.g. types I and III) |
| 2. Second harmonic generation | SHG | Cyan | Fibrillar collagen | Fibrillar collagen (e.g. types I and III) ex: 850 nm; em: 425 nm |
| 3. Isosurface mapping | ||||
| a. Signal | Cyan | Collagen SHG | Fibrillar collagen (e.g. types I and III) | |
| Green | siGLO | Trabecular meshwork | ||
| Red | VE-cadherin-td-Tomato | Blood vessel endothelium | ||
| b. Signal voids | Yellow | Schlemm’s canal lumen | Distal drainage tract | |
| Collector channel lumen | Distal drainage tract | |||
| Intrascleral plexus lumen | Distal drainage tract | |||
| 4. Immunohistochemistry | IHC | Red | F-actin | Contractility marker |
| (by one-photon, confocal microscopy) | Green | alpha-smooth muscle actin | Contractility marker with smooth muscle identity | |
| Caldemson | Contractility marker with smooth muscle identity | |||
| Calponin | Contractility marker with smooth muscle identity | |||