Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 6;7:17063. doi: 10.1038/s41598-017-16624-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The sequence, expression and cellular localization of amphioxus PIAS. (a) Phylogenetic analysis of PIAS proteins based on the highly conserved RLD domain. The tree was inferred using MEGA7 with the Maximum Likelihood method and 500 bootstrap tests. (b) The protein architecture of PIAS family members. The sequence length is indicated. (c,d) qRT-PCR analysis of the time course of bPIASv1 and v2 mRNA expression dynamics after challenge with LTA and LPS. Note that the 0 h of treatment is equivalent to the unchallenged animals. (e,f) qRT-PCR analysis of bPIASv1 and v2 mRNA expression in different tissues. (g,h) qRT-PCR analysis of dynamic bPIASv1 and v2 mRNA expression during amphioxus embryogenesis. The data were expressed as a ratio between bPIASv1 or v2 mRNA and the mRNA expression level of GAPDH. (i) The truncated mutants of bPIAS used in this study. (j) The subcellular localization of full-length bPIASv1 and v2 and the associated truncated mutants. The scale bar indicates 10 µm.