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. 2017 Dec 6;7:17082. doi: 10.1038/s41598-017-17357-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NaF exposure affects cell cycle progression in porcine oocyte. (a) The percentage of oocytes undergoing GVBD after NaF treatment for 24 or 30 h. (b) Representative confocal images of porcine oocytes in various maturation phases; scale bar = 10 μm; blue, DNA; green, α-tubulin. (c) Percentages of cells in various meiotic maturation phases were calculated at 44 h. (d) Time-lapse images obtained after microinjecting GV-stage oocytes with histone H2B–mCherry cRNA; Scale bar = 20 μm. ATI: anaphase/telophase I; GV: germinal vesicle; GVBD: germinal vesicle breakdown; MII: metaphase II. Data are presented as mean ± SD of three independent experiments. Statistically significant differences are indicated by asterisks (*p < 0.05).