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. 2017 Dec 6;7:17082. doi: 10.1038/s41598-017-17357-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NaF exposure induces defects in spindle assembly in porcine oocytes. (a) Representative spindle morphologies of oocytes in the MI stage. Control oocytes showed normal spindle morphology, whereas NaF-treated oocytes showed various spindle defects (arrows); scale bar = 10 μm; blue, DNA; green, α-tubulin. (b) Percentages of NaF-treated oocytes in the MI stage showing aberrant spindle morphology. (c) Spindle positioning in oocytes in the MI stage. Spindles in control oocytes were located almost peripherally, whereas those in NaF-treated oocytes were located almost centrally; scale bar = 20 μm. (d) Quantification of the distance between the spindle and cell cortex (length/diameter) in control and NaF-treated oocytes. Data are presented as mean ± SD of three independent experiments. Statistically significant differences are indicated by asterisks (**p < 0.01).