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. 2017 Dec 6;7:17082. doi: 10.1038/s41598-017-17357-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NaF exposure effects on ROS level, GSH level, and cathepsin B activity in porcine oocytes. (a) NaF exposure increased ROS generation. (b) Relative fluorescence intensity of ROS in control and NaF-treated oocytes. (c) NaF exposure decreased GSH level. (d) Relative fluorescence intensity of GSH in control and NaF-treated oocytes. (e) NaF exposure increased cathepsin B activity. (f) Relative fluorescence intensity of cathepsin B in control and NaF-treated oocytes. Scale bar = 100 μm. Data are presented as mean ± SD of three independent experiments. Statistically significant differences are indicated by asterisks (*p < 0.05).