Figure 2.

Growth curve of 50 mutants and a wild type S. Typhimurium in LB, H₂O₂L and H₂O₂H. The lag phase time, growth rate, and maximum OD₆₀₀ of the individual S. Typhimurium mutants and the wild type were measured in LB media supplemented with no H₂O₂ (LB), 2.5 mM H₂O₂ (H₂O₂L) or 3.5 mM H₂O₂ (H₂O₂H). Overnight culture of each of the mutants and wild type was diluted 1:200 in LB, H₂O₂L and H₂O₂H. The cultures were incubated at 37 °C for 24 h in a 96-well plate and the OD₆₀₀ was recorded every 10 min. The lag phase time, growth rate, and maximum OD₆₀₀ were calculated and shown here as a graphical representation. The pale pink color indicates a short lag phase time, a high growth rate, and a high OD₆₀₀. The red color indicates that the bacteria stayed in a lag phase, growth rate was close to a zero, and OD₆₀₀ of the culture did not increase during the 24 h time of the assays. The data of this figure can be found in Table S4.