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. 2017 Dec 6;7:17047. doi: 10.1038/s41598-017-17270-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vitamin K2 lowers ROS release. (A) Confocal microscopy showed increased ROS in Lymph gland of heix mutants, reduced by VK2 treatment. (B) Confocal microscopy showed increased ROS in Brain of heix mutants, reduced by VK2 treatment. Both lesions responded significantly to treatment. DAPI stain (blue) and ROS staining (CM-H2DCFDA) (green). Control media contain 50 mM Ethanol (EtOH) and treatment media contain 50 mM VK2. Means normalized to control (Canton S). Error bars indicate SEM. Analysis of variance (AVOVA/Dunnett: *P < 0.05, **P < 0.01, ***P < 0.001).