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. 2017 Dec 6;7:17070. doi: 10.1038/s41598-017-17381-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Interaction and localization of TPPP/p25 and SIRT2 in living HeLa cells as detected by immunofluorescence microscopy coupled with BiFC technology. (a) Scheme of the applied BiFC constructs. (b) Co-localization of the TPPP/p25-SIRT2 complex (green) with the MT network (red). MT network was stained with Alexa546, nuclei was counterstained with DAPI (blue). Scale bar: 10 μm. (c) BiFC signal (green) of the assembly of Venus^N-TPPP/p25 and Venus^C-SIRT2 and the effect of unlabelled TPPP/p25, α-synuclein and MZ25 was quantified as described in the Materials and Methods. The data are presented as mean ± SD, in each case at least 90 cells were analysed. *p = 2.45E-21 when control (BiFC) cells were compared with those co-transfected with unlabelled TPPP/p25 and *p = 6.76E-06 with unlabelled α-synuclein (two-sided, unpaired Student’s t-test).