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. 2017 Dec 6;7:17095. doi: 10.1038/s41598-017-16609-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microvesicles and exosomes have similar sizes and characteristics. Nanoparticle Tracking Analysis (NTA) showed overlapping size distributions of exosomes (a) and microvesicles (MV) (b), a mean diameter of 153 nm for exosomes and 170 nm for MVs (p = 0.026, c) and equal numbers of vesicles released per cell (d). Results are pooled of three independent experiments conducted on in total six separate preparations per vesicle type. Five 60 second recordings per sample were batch analysed. Transmission electron microscopy showed typical characteristics of exosomes (e) and similar, but generally larger sizes of MVs (f). Size bars (in e and f) are 200 nm per segment (black or white).