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. 2017 Dec 6;7:17056. doi: 10.1038/s41598-017-17316-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Particle sizes, zeta potentials and transfection efficacies of PF14 analogues with modified charge and hydrophobicity. (a) Hydrodynamic diameter and zeta potential of complexes prepared with PF14 analogues and pDNA. (b) The pDNA transfection efficacy PF14 and its analogs was evaluated in cultured CHO cells. The most efficient transfection was mediated by the vectors with the highest charge density (PF6 > PF14-O > PF14 > jetPEI). Decrease of the carbon chain length of PF14 also decreased the transfection. Statistical analysis is presented in the Supplementary Data.