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. 2017 Dec 6;7:17056. doi: 10.1038/s41598-017-17316-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The stability of the complexes in challenging environments. (a) Transfection efficacy is assessed after DNase I treatment. The particles are formed at N/P2, digested with DNase and used for the transfection in CHO cells. jetPEI was formed at N/P8. (b) The stability of the complexes against proteinase K was evaluated by plotting the amount of accessible pDNA in time (assessed with fluorescent DNA intercalating dye). The peptide complexes were formed at N/P2 (unless indicated otherwise), jetPEI was formed at N/P8.