Fig. 3.

Effect of combination of protein kinase inhibitors on cell cycle protein in melanoma cells. Melanoma cell lines: a WM793, b Lu1205 were treated with combination of protein kinase inhibitors. Cell cycle protein expression was analyzed by western blot. Densitometry analyses of western blot were performed on raw volume (sum of intensities of bound-volume calculated from the area of the peak) using SynGene Gene Tools version 4.03.0 (Synoptics Ltd Beacon House, Nuffield Road Cambridge, CB4 1TF, UK). Densitometry was used to normalize to control (melanoma cells untreated with protein kinase inhibitors). Presented are representative of at least three independent experiments with similar results