Table 3.
Glucuronides produced by incubation of different DON metabolites with rat or human liver microsomes
| Substrate | Type of microsome | Glucuronides |
|---|---|---|
| DON | RLM | DON-3-GlcAc (comp 6, 100%) ≫ DON-8,15-hemiketal-8-GlcAc (comp 5, 5.2%) > DON-15-GlcAc (comp 7, 2.0%) > unknown DON-GlcAc (comp 2, 0.3%) > iso-DON-3-GlcAc (comp 9, 0.2%) ~iso-DON-8-GlcAc (comp 1, 0.1%) |
| DON | HLM | DON-15-GlcAc (comp 7, 100%) > DON-3-GlcAc (comp 6, 23%) |
| DOM | RLM | DOM-3-GlcAc (comp 12, 100%) ≫ DOM-15-GlcAc (comp 13, 2.6%) > unknown DOM-GlcAc b (comp 10, 0.8%) > unknown DOM-GlcAc a (comp 4, 0.3%) > iso-DOM-3-GlcAc (comp 14, <0.2%) ~iso-DOM-8-GlcAc (comp 3, <0.2%) |
| DOM | HLM | DOM-15-GlcAc (comp 13, 100%) ≫ DOM-3-GlcAc (comp 12, 7.7%) |
| Iso-DON | RLM | iso-DON-8-GlcAc (comp 1, 100%) > iso-DON-3-GlcAc (comp 9, 13%) > iso-DON-15-GlcAc (8.0%) |
| Iso-DON | HLM | iso-DON-15-GlcAc (100%) > iso-DON-3-GlcAc (comp 9, 29%) |
| Iso-DOM | RLM | iso-DOM-8-GlcAc (comp 3, 100%) > iso-DOM-3-GlcAc (comp 14, 54%) ~iso-DOM-15-GlcAc (54%) |
| Iso-DOM | HLM | iso-DOM-15-GlcAc (100%) ≫ iso-DOM-3-GlcAc (comp 12, 3.1%) |
In brackets: relative intensities (values given relative to the major glucuronide in each assay) based on relative peak areas of the fragment m/z 113.0; comp: compound