Table 4.
Semi-preparative HPLC isolation of glucuronides and parent compounds from microsome assay solutions. ref.: reference
| Compound numbera | Compound name (method of identification) |
Production | Fractionsb | Collection time (min) |
|---|---|---|---|---|
| 1 | Iso-DON-8-GlcAc (NMR) | DON RLM | 6 | 3.5–3.7 |
| 2 | Unknown DON-GlcAc | DON RLM | 9–11 | 4.1–4.7 |
| 5 | DON-8.15-hemiketal-8-GlcAc (ref. standard) | DON RLM | 15, 16 | 5.3–5.7 |
| 6 | DON-3-GlcAc (ref. standard) | DON RLM | 18, 19 | 5.9–6.3 |
| 9 | Iso-DON-3-GlcAcc (NMR) | DON RLM | 25 | 7.3–7.5 |
| 7 | DON-15-GlcAcd (literature) | DON HLM | 20 | 6.3–6.5 |
| 8 | DON (ref. standard) | DON RLM, DON HLM | 22 | 6.7–6.9 |
| 11 | Iso-DONe (NMR) | DON RLM, DON HLM | 26 | 7.5–7.7 |
| 3 | Iso-DOM-8-GlcAcf (NMR) | DOM RLM | 8 | 3.9–4.1 |
| 4 | Unknown DOM-GlcAc a | DOM RLM | 11 | 4.5–4.7 |
| 10 | Unknown DOM-GlcAc b | DOM RLM | 23 | 6.9–7.1 |
| 12 | DOM-3-GlcAc (NMR) | DOM RLM | 27, 28 | 7.7–8.1 |
| 13 | DOM-15-GlcAc (NMR) | DOM HLM | 30 | 8.3–8.5 |
| 14 | Iso-DOM-3-GlcAcg (NMR) | DOM RLM | 36 | 9.5–9.7 |
| 15 | DOM (ref. standard) | DOM RLM, DOM HLM | 34 | 9.1–9.3 |
| 16 | Iso-DOMh (NMR) | DOM RLM, DOM HLM | 37 | 9.7–9.9 |
aNumber of the compound in Fig. 2
bFractions containing pure compound unless stated otherwise
c−hOnly collected in mixed fractions. Impurities: c DON and iso-DON, d DON-3-GlcAc, e iso-DON-3-GlcAc (DON RLM), DON (DON HLM), f unknown DOM-GlcAc a, g DOM, h DOM