Skip to main content
. 2017 Jul 10;38(12):1632–1641. doi: 10.1038/aps.2017.78

Figure 6.

Figure 6

ABT-263-induced autophagy counteracted its inhibitory activity on cell viability and colony formation. (A) The autophagy inhibitor CQ attenuated the ABT-263-induced loss of cell viability in human esophageal cancer cell lines. Cells were treated with ABT-263 (10 μmol/L), CQ (10, 50 μmol/L) or both for 24 h. Cell viability was assessed with an MTT assay. (B) The autophagy inhibitor CQ sensitizes human esophageal cancer cells to ABT-263. EC109, HKESC-2 and CaES-17 cells were allowed to grow for 10 d after the withdrawal of ABT-263 (EC109, 10 μmol/L, HKESC-2, 6 μmol/L, CaES-17, 10 μmol/L) and CQ (EC109, 15 μmol/L, HKESC-2, 10 μmol/L, CaES-17, 10 μmol/L) alone or both at 48 h. The colonies were stained with hematoxylin, and their numbers are displayed in the charts. Data are presented as the mean±SEM of three independent experiments. NS indicates “no significant difference” (P>0.05). *P<0.05, **P<0.01, ***P<0.001 compared with the control.