Fig. 2.

Integrin β1 Y783A kidneys are small with evidence of abnormal UB development. (A-F) Kidneys were isolated from embryos of wild-type and Y783A mice at E12.5 (A-D). The branching UB is indicated with arrows (A,B). Scale bar: 50 μm. In situ hybridization of whole-mount kidneys were performed with antisense to Wnt11 (C,D). Scale bars: 100 μm. Y785A kidneys at E15.5 (E,F) and E17.5 (G,H) were small and had less UB branching. Scale bars: 100 μm in E,F; 200 μm in G,H. (I,J) The CDs designated by staining positive for the lectin Dolichos biflorus were dilated in E17.5 kidneys. Scale bars: 50 μm. At least six embryos at each time point were examined.