Fig. 3.

Integrin β1 Y783A kidneys have a branching morphogenesis defect. (A-C) Whole-mount dissections of the urogenital tract reveal that the length of the E15.5 Y783A kidneys was significantly less than in wild-type mice. (D-H) Whole mounts of E15.5 kidneys, stained with E-cadherin and imaged with confocal microscopy show a significant branching defect. At least six mice of each genotype were examined. Scale bars: 100 μm in D-G. (I,J) High-powered images of Ki67 staining in E15.5 Y783A and wild-type mice showed decreased UB cell proliferation in Y783A mice at E15.5. Scale bars: 100 μm. (K) The number of Ki67-positive cells was quantified and expressed as the mean±s.d. of five high-power fields in five mice. *P<0.05 (between wild-type and Y783A mutant mice).