Fig. 7.

Talin 1/2-null mice are anephric due to the arrest of UB growth at E12.5. (A) Newborn talin 1/2-null mice were anephric, despite normal localization of the adrenal gland (asterisks). The arrows in the wild-type mouse indicate the kidneys. Twenty mice were investigated with similar results. (B-E) In situ hybridization of Ret was performed on whole mounts of E11 and E 12.5 kidneys. Induction of the UB at E11 was delayed in the talin-null kidneys (B,C) and it never progressed beyond the T shape in the E12.5 kidneys (D,E). Scale bars: 100 μm. (F,G) Whole mounts of E12.5 kidneys from wild-type and talin 1/2-null mice were stained with E-cadherin and visualized by confocal microscopy. Scale bars: 100 μm. At least three embryos of each phenotype were investigated with similar results.