Fig. 4.

c-Myc promotes Fam19a5-GRE reporter activity in vitro. (A) Sequence fragments from the Fam19a5-GRE highlighting the consensus Lef/Tcf (red)- and Myc (blue)-binding sites. (B) Graph of luciferase reporter activity from HEK 293 cells transfected with a Fam19a5-GRE luciferase reporter and treated with LiCl at different concentrations. (C) Graph of luciferase activity from HEK 293 cells transfected with wild-type or various mutant Fam19a5-GRE reporters. Some cells were co-transfected with a full length c-Myc expression plasmid as indicated. The media was replaced with fresh media (black bars) or media supplemented with 10 mM LiCl (gray bars) 24 h after transfection and luciferase activity was measured 18 h later. Data are represented as mean+s.e.m.