Fig. 5.

Myc is necessary for Fam19a5 reporter activity and Wnt9b target gene expression in organ culture. E11.5 Fam19a5-GRE kidneys were dissected out and cultured for 48 h in media supplemented with DMSO (A,F-H), the Wnt production inhibitor IWP2 (B), a Myc inhibitor (C,I-K), a STAT1 inhibitor (D) or a STAT3 inhibitor (E). Cultured kidneys were fixed and stained with X-gal (A-E), antisense mRNA for Cited1 (F,I) or Fam19a5 (G,J), or sectioned and stained with antibodies to β-gal (bgal, green), Six2 (red) and TO-PRO-3 (blue) (H,K). Arrows indicate the nephron progenitor cells and ureteric bud is outlined. Scale bars: 30 µm.