Fig. 1.
Targeted disruption of the mouse β-TrCP2 gene. (A) Schematic representation of wild-type, floxed and deleted alleles of the mouse β-TrCP2 locus. LoxP sites are shown as triangles. Deletion of exon 5 by Cre recombinase results in a frameshift that generates a premature stop codon. The positions of PCR primers (F, R1, R2) for genotyping are indicated. (B) Genomic PCR analysis for the testis of β-TrCP2 CKO or β-TrCP2F/F mice at the indicated ages. The remaining floxed band in Stra8-Cre animals is due to unrecombined alleles in somatic cells of the testis. (C) RT-qPCR analysis of β-TrCP2 mRNA abundance in the testis of β-TrCP2 CKO mice expressed relative to that for age-matched β-TrCP2F/F (control) mice. Data are mean±s.e.m. for three mice of each genotype. *P<0.05 versus corresponding control (two-way ANOVA followed by Tukey's post-hoc test).