Table 2.

Trial sample collection schedule

Perfusate samples	Hepatic arterial and hepatic venous biochemistry (point of care)	Preperfusion Every 30 min during perfusion	Cobas point-of-care desktop analyser
	Perfusate supernatant	Preperfusion Every 15 min for first hour Every hour thereafter	5×1 mL aliquots Stored at −80°C
Liver samples	Liver biopsy	L1 Preperfusion L2 After 4 hours L3 at end of perfusion* L4 Postreperfusion	16 G core needle biopsy Divided into segment for formalin, segment for frozen and piece for electron microscopy
	Common bile duct	CBD1 Preperfusion CBD2 Postreperfusion	Formalin
Bile samples	(If produced)	B2 sample at 2 hours B4 sample at 4 hours B6 sample at 6 hours	Total volume recorded and 2 mL samples snap frozen at these time-points
Patient samples	Biochemistry haematology clotting	Visits 1, 2, 3, 4, extended follow-up	Standard of care
	Serum, plasma, mononuclear cells (PBMC)	Visit 1 (preoperative (postinduction of anaesthesia), postreperfusion day 4 postoperative) Visits 2, 3, 4,	Additional research samples
	Urine	Visit 1 (pre-operative [post-induction], postreperfusion Day four post-op)	Additional research samples

*If lasting longer than 6 hours.

PBMC, peripheral blood mononuclear cell.