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. 2017 Nov 25;7(11):e018109. doi: 10.1136/bmjopen-2017-018109

Table 2.

MLVA primers and fluorescence dyes used in each multiplex reaction

VNTR Fluorochromes Primer sequences (5’–3’) Allele size range (bp)
Multiplex 1
CE 1


 Asp 167 F:
R:		 ATTO565- TGAGATGGTTAACTTACGTAGCGC
CGCTCCCACCGTTACCAAC		 graphic file with name bmjopen-2017-018109ilf01.jpg
Asp 330 F:
R:		 ATTO550- ATCTGGTCGCGAAATTCCTCT
TCTTCGGCCTTTTCATCCC		 graphic file with name bmjopen-2017-018109ilf02.jpg
Asp 345 F:
R:		 Yakima Yellow- TCTCCAACCCTTCGGACG
GCCGGAAGAGCATGAAGACA		 graphic file with name bmjopen-2017-018109ilf03.jpg
Asp 443 F:
R:		 ATTO565- AAGCTTCGTCTGGCGAAGAG
GCACGTGTACGGTGTTCCTG		 graphic file with name bmjopen-2017-018109ilf04.jpg
Asp 446 F:
R:		 6-FAM- CGATCATGTTTGCCTGAGGA
CCGACAGCATCGAGCAACTA		 graphic file with name bmjopen-2017-018109ilf05.jpg
Multiplex 2
CE 2		 Asp 20 F:
R:		 6-FAM- GGGAAGAGAGGAACCGATCC
CGCAGTGGGCAGTTTGAAT		 graphic file with name bmjopen-2017-018109ilf06.jpg
Asp 165 F:
R:		 Yakima Yellow- TGATGGGCCGCAGTCG
GCACCTGCTTGTCGATTCGT		 graphic file with name bmjopen-2017-018109ilf07.jpg
Asp 202 F:
R:		 ATTO565- AGGATCACTGCCCTCAACCC
CCGAAATCCGCGGGA		 graphic file with name bmjopen-2017-018109ilf08.jpg
Asp 204bis F:
R:		 ATTO565- ATTGGGAAGAGACGGGGTAT
GTCCTCACTTTTGCCTTGGT		 graphic file with name bmjopen-2017-018109ilf09.jpg
Asp 252 F:
R:		 ATTO550- CAGATTGGAGACACGAAGCG
ACCACGGATTGCCAAGGA		 graphic file with name bmjopen-2017-018109ilf10.jpg

6-FAM, 6-carboxyfluorescein; CE, capillary electrophoresis; F, forward; MLVA, multiple-locus variable-number tandem-repeat analysis; R, reverse; VNTR, variable-number tandem repeat.