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. 2017 Dec 7;7:17156. doi: 10.1038/s41598-017-16009-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of Cdx2 functional targets in vivo from E3.5 Knockout blastocysts. (a) qPCR analysis of E3.75 blastocysts derived from Cdx2 heterozygous intercrosses. Each bars represents one blastocyst. All expression levels are normalized against endogenous control Actb. The order of the embryos is sorted according to Cdx2 expression. (b) Hierarchical clustering of expression profiles of all analyzed individual blastocysts. (c) Expression correlation map of different genes to Cdx2. X-axis indicates gene correlation with Cdx2 in single cells harvested from ~E3.75 wild type embryos. Y-axis indicates gene correlation with Cdx2 in E3.75 blastocysts harvested from Cdx2+/− intercrosses. See text for discussion of cluster 1, 2, and 3.