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. 2017 Dec 7;7:17119. doi: 10.1038/s41598-017-17227-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Photograph of an Indian saw scaled viper (Echis carinatus). Photograph courtesy of Romulus Whitaker, Nov, 2010. (b) Fractionation of ECV (2.0 mg dry weight) on a Shodex KW- 803 gel filtration column coupled to Dionex Ultimate 3000 UHPLC system (Thermo Fisher Scientific, USA). The flow rate was maintained at 10 ml/h at room temperature (~23 °C) and fractions of 0.25 ml were collected. (c) SDS-PAGE analysis of crude ECV. Lane 1, 2 and 3 represents crude ECV (non-reduced), protein molecular markers and crude ECV (reduced) respectively.