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. 2017 Dec 7;8:1987. doi: 10.1038/s41467-017-02031-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Identification of a DcUGT glucosylating FK in vitro. Microsomes of D. coccus and of yeast expressing Strep-tagged DcUGT2, DcUGT5 and pYES-DEST52 vector, respectively, were incubated with [¹⁴C]UDP-glucose and with/without flavokermesic acid. As a control, D. coccus microsomes were incubated with the asperthecin aglucone and [¹⁴C]UDP-glucose. Formed products were separated by TLC. a Aglucone substrates tested in the in vitro glucosylation assay. b TLC-separated [¹⁴C] products formed in vitro and viewed by phosphorimaging. FK flavokermesic acid, CA carminic acid